pIVT-R2Tg-RTmut
(Plasmid
#238106)
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PurposeFor IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIVT
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Vector typeFor IVT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameReverse transcriptase mutant R2Tg, mammalian codon optimized
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SpeciesH. sapiens (human), M. musculus (mouse); T. guttata (zebra finch)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVT-R2Tg-RTmut was a gift from Feng Zhang (Addgene plasmid # 238106 ; http://n2t.net/addgene:238106 ; RRID:Addgene_238106) -
For your References section:
Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons. Edmonds KK, Wilkinson ME, Strebinger D, Chen H, Lash B, Schaefer CC, Zhu S, Liu D, Zilberzwige-Tal S, Ladha A, Walsh ML, Frangieh CJ, Vaz Reay NA, Macrae RK, Wang X, Zhang F. Nat Commun. 2025 Jul 2;16(1):6079. doi: 10.1038/s41467-025-61321-z. 10.1038/s41467-025-61321-z PubMed 40603868