pTmpl-miniDonor-GFP
(Plasmid
#238107)
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PurposeMini donor expressing GFP, used for PCR to generate IVT template.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDIRECT
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMV-GFP-syntheticPA flanked by R2Tg UTRs and 28S sequence
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTmpl-miniDonor-GFP was a gift from Feng Zhang (Addgene plasmid # 238107 ; http://n2t.net/addgene:238107 ; RRID:Addgene_238107) -
For your References section:
Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons. Edmonds KK, Wilkinson ME, Strebinger D, Chen H, Lash B, Schaefer CC, Zhu S, Liu D, Zilberzwige-Tal S, Ladha A, Walsh ML, Frangieh CJ, Vaz Reay NA, Macrae RK, Wang X, Zhang F. Nat Commun. 2025 Jul 2;16(1):6079. doi: 10.1038/s41467-025-61321-z. 10.1038/s41467-025-61321-z PubMed 40603868