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His6-tev-G(TTR A25T/I26V)
(Plasmid #238117)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21d
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5440
  • Total vector size (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    462
  • Mutation
    A25T/I26V
  • Entrez Gene
    TTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
  • Promoter T7
  • Tag / Fusion Protein
    • His6tev-G (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jeffery Kelly lab; we made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid in Zhang et al. 2014 ACS Chem Biol (https://doi.org/10.1021/cb5004477).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-tev-G(TTR A25T/I26V) was a gift from Peter Wright (Addgene plasmid # 238117 ; http://n2t.net/addgene:238117 ; RRID:Addgene_238117)

  • For your References section:

    Probing the Dissociation Pathway of a Kinetically Labile Transthyretin Mutant. Sun X, Ferguson JA, Leach BI, Stanfield RL, Dyson HJ, Wright PE. J Am Chem Soc. 2024 Jan 10;146(1):532-542. doi: 10.1021/jacs.3c10083. Epub 2023 Dec 22. 10.1021/jacs.3c10083 PubMed 38134439
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