pRRL-SFFV_V5-CHIC2-C6S_Tomato
(Plasmid
#238128)
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PurposeExpresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRL
- Backbone size w/o insert (bp) 8017
- Total vector size (bp) 8557
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Vector typeMammalian Expression
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Selectable markersTomato
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV5-tagged CHIC2 with C6S mutation
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SpeciesH. sapiens (human)
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Insert Size (bp)540
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MutationChanged cysteines from amino acids 102-109 to serines.
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GenBank ID26511
- Promoter SFFV
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Tag
/ Fusion Protein
- V5 (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer SFFV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript performed this cloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL-SFFV_V5-CHIC2-C6S_Tomato was a gift from Arlene Sharpe (Addgene plasmid # 238128 ; http://n2t.net/addgene:238128 ; RRID:Addgene_238128)