pRRL-SFFV_STUB1-UBOXdel-V5_Tomato
(Plasmid
#238129)
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PurposeExpresses V5-tagged STUB1 (with UBOX deletion) with Tomato selectable marker.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRRL
- Backbone size w/o insert (bp) 8017
- Total vector size (bp) 8761
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Vector typeMammalian Expression
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Selectable markersTomato
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV5-tagged-STUB1
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SpeciesM. musculus (mouse)
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MutationDeleted UBOX domain (amino acids 224-294)
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GenBank ID56424
- Promoter SFFV
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer SFFV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript performed the cloning.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL-SFFV_STUB1-UBOXdel-V5_Tomato was a gift from Arlene Sharpe (Addgene plasmid # 238129 ; http://n2t.net/addgene:238129 ; RRID:Addgene_238129)