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pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse
(Plasmid #238133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238133 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLCKO
  • Backbone size w/o insert (bp) 9153
  • Total vector size (bp) 10530
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMEM106B
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1377
  • GenBank ID
  • Entrez Gene
    Tmem106b (a.k.a. 2310036D22Rik, 5830455K21Rik, 6430519M21Rik)
  • Promoter CMV
  • Tag / Fusion Protein
    • Blasticidin-P2A-3xHA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse was a gift from Dirk Daelemans (Addgene plasmid # 238133 ; http://n2t.net/addgene:238133 ; RRID:Addgene_238133)

  • For your References section:

    TMEM106B is a receptor mediating ACE2-independent SARS-CoV-2 cell entry. Baggen J, Jacquemyn M, Persoons L, Vanstreels E, Pye VE, Wrobel AG, Calvaresi V, Martin SR, Roustan C, Cronin NB, Reading E, Thibaut HJ, Vercruysse T, Maes P, De Smet F, Yee A, Nivitchanyong T, Roell M, Franco-Hernandez N, Rhinn H, Mamchak AA, Ah Young-Chapon M, Brown E, Cherepanov P, Daelemans D. Cell. 2023 Aug 3;186(16):3427-3442.e22. doi: 10.1016/j.cell.2023.06.005. Epub 2023 Jul 7. 10.1016/j.cell.2023.06.005 PubMed 37421949
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