pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666 Sequences (1)

Addgene Sequences:

Full (1)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

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> Addgene NGS Result gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggt ctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgac agttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatacc gcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaga agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttgg tatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaa gcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggtta tggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactc aaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaa ggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttt tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcg acacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtc tcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg aaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatct gctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgc gagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttagg cgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaat agtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaa tggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagta acgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtac atcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcatta tgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtc tccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaa caactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttt tgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaaccca ctgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctg gtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggac ttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaa gaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgg gtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccaggg ggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatc ctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacagg atcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagata aaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaag cggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaa tataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagag aaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgc agcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttg ctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaa gaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaact catttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcac acgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaat cgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattg gtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggttta agaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcaga cccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacag agacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtatt catccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataata gcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattaca gggacagcagagatccagtttggttagtaccgggcccgctctagagatccgacgcgccatctctaggccc gcgccggccccctcgcacggacttgtgggagaagctcggctactcccctgccccggttaatttgcatata atatttcctagtaactatagaggcttaatgtgcgataaaagacagataatctgttctttttaatactagc tacattttacatgataggcttggatttctataacttcgtatagcatacattatacgaagttataaacagc acaaaaggaaactcaccctaactgtaaagtaattgtgtgttttgagactataagtatcccttggagaacc accttgttgGAAGTTGTGTACAAACTGCGgtttaagagctaagctggaaacagcatagcaagtttaaata aggctagtccgttatcaacttggccgctttaaggccggtcctagcaaggccaagtggcaccgagtcggtg ctttttttctcgagtactaggatccattaggcggccgcgtggataaccgtattaccgccatgcatgtgcc cgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccg gtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccga gggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgcc agaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgc cttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtggga gagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctgg ggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccattt aaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatct gcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcg gcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctc tggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagt tgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcg ggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtg actccacggagtaccgggcgccgtccaggcacctcgattagttctcgcgcttttggagtacgtcgtcttt aggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggcca gcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagc ctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagctagcccaccatgaccgagtac aagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcg ccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaaga actcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcg gtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagt tgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcc cgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtg ctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacc tccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctg gtgcatgacccgcaagcccggtgccggatcgggagagggcagaggaagtctgctaacatgcggtgacgtc gaggagaatcctggcccaccggtcgccaccATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGC CCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGA TGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC CTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACT TCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTA CAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGAC TTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCA TGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGT GCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGT TCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAAgaattcgtcgagggacctaa taacttcgtatagcatacattatacgaagttatacatgtttaagggttccggttccactaggtacaattc gatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaact atgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtat ggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtc aggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacct gtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcct tgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcg tcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtccctt cggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcg ccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgatcg agacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggcta gaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttaca aggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacg aagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacaca ccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagaga aggtagaagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatggatga cccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctg catccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactaggga acccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtga ctctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagcatgtgagcaaaa ggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctg acgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggc gtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcc tttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcg ttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaacta tcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagc agagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaa cagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccgg caaacaaaccaccgctggtagcggtggttttttt

pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666 Sequences (1)

Full Sequences from Addgene (1)

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