pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668 Sequences (1)

Full Sequences from Addgene (1)

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  > Addgene NGS Result gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggt ctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcac ctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgac agttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatacc gcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaga agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagtt cgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttgg tatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaa gcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggtta tggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactc aaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataat accgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaa ggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttt tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcg acacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtc tcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccg aaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatct gctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgc gagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttagg cgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaat agtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaa tggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagta acgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtac atcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcatta tgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtc tccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaa caactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttt tgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaaccca ctgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctg gtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggac ttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaa gaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgg gtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccaggg ggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatc ctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacagg atcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagata aaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaag cggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaa tataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagag aaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgc agcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttg ctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaa gaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaact catttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcac acgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaat cgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattg gtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggttta agaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcaga cccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacag agacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtatt catccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataata gcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattaca gggacagcagagatccagtttggttagtaccgggcccgctctagagatccgacgcgccatctctaggccc gcgccggccccctcgcacggacttgtgggagaagctcggctactcccctgccccggttaatttgcatata atatttcctagtaactatagaggcttaatgtgcgataaaagacagataatctgttctttttaatactagc tacattttacatgataggcttggatttctataacttcgtatagcatacattatacgaagttataaacagc acaaaaggaaactcaccctaactgtaaagtaattgtgtgttttgagactataagtatcccttggagaacc accttgttgGACGATGGAAGGGAGCCTCGgtttaagagctaagctggaaacagcatagcaagtttaaata aggctagtccgttatcaacttggccgctttaaggccggtcctagcaaggccaagtggcaccgagtcggtg ctttttttctcgagtactaggatccattaggcggccgcgtggataaccgtattaccgccatgcatgtgcc cgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccg gtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccga gggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgcc agaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgc cttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtggga gagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctgg ggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccattt aaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatct gcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcg gcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctc tggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagt tgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcg ggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtg actccacggagtaccgggcgccgtccaggcacctcgattagttctcgcgcttttggagtacgtcgtcttt aggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggcca gcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagc ctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagctagcccaccatgaccgagtac aagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcg ccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaaga actcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcg gtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagt tgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcc cgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtg ctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacc tccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctg gtgcatgacccgcaagcccggtgccggatcgggagagggcagaggaagtctgctaacatgcggtgacgtc gaggagaatcctggcccaccggtcgccaccATGgtgtctaagggcgaagagctgattaaggagaacatgc acatgaagctgtacatggagggcaccgtggacaaccatcacttcaagtgcacatccgagggcgaaggcaa gccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgac atcctggctactagcttcctctacggcagcaagaccttcatcaaccacacccagggcatccccgacttct tcaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgac cgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttc acatccaacggccctgtgatgcagaagaaaacactcggctgggaggccttcaccgagacgctgtaccccg ctgacggcggcctggaaggcagaaacgacatggccctgaagctcgtgggcgggagccatctgatcgcaaa cgccaagaccacatatagatccaagaaacccgctaagaacctcaagatgcctggcgtctactatgtggac tacagactggaaagaatcaaggaggccaacaacgagacctacgtcgagcagcacgaggtggcagtggcca gatactgcgacctccctagcaaactggggcacaagcttaatTAAgaattcgtcgagggacctaataactt cgtatagcatacattatacgaagttatacatgtttaagggttccggttccactaggtacaattcgatatc aagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttg ctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggcttt cattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaa cgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagc tcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccg ctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtccttt ccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccc tcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcg ccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgatcgagacct agaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagca caagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcag ctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagaca agatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacacaccaggg ccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagagaaggtag aagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatggatgacccgga gagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctgcatccg gactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccac tgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctgg taactagagatccctcagacccttttagtcagtgtggaaaatctctagcagcatgtgagcaaaaggccag caaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagc atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttcc ccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctc ccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgct ccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtct tgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcg aggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtat ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaaca aaccaccgctggtagcggtggttttttt