pGFP_4
(Plasmid #238193)

• Purpose: (Empty Backbone) Empty lentiviral vector for expressing protein sequences fused to GFP-4mer_oligomerization_domain, under a TRE3GV promoter
• Depositing Lab: Feng Zhang
• Publication: Characterizing protein sequence determinants of nuclear condensates by high-throughput pooled imaging with CondenSeq (unpublished)

Backbone

• Vector backbone: pLenti CMV rtTA3 Blast (w756-1) (Addgene #26429)
• Backbone manufacturer: Eric Campeau
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin
• Tags / Fusion Proteins:
  • GFP-4mer (N terminal on backbone)
  • SV40 (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • GFP-4mer (N terminal on backbone)
  • SV40 (N terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry