pMW357
(Plasmid
#238204)
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PurposeExpresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTaTasR
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Alt namehypothetical protein DWQ19_11745
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Alt nameRDJ35482.1
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SpeciesThermoproteota archaeon
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Insert Size (bp)1820
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMW357 was a gift from Feng Zhang (Addgene plasmid # 238204 ; http://n2t.net/addgene:238204 ; RRID:Addgene_238204) -
For your References section:
TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses. Faure G, Saito M, Wilkinson ME, Quinones-Olvera N, Xu P, Flam-Shepherd D, Kim S, Reddy N, Zhu S, Evgeniou L, Koonin EV, Macrae RK, Zhang F. Science. 2025 Feb 27:eadv9789. doi: 10.1126/science.adv9789. 10.1126/science.adv9789 PubMed 40014690