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Addgene

pMIIS1011
(Plasmid #238212)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238212 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ParTasR
  • Alt name
    hypothetical protein WC565_07165
  • Alt name
    MFA5053820.1
  • Species
    Parcubacteria group bacterium
  • Insert Size (bp)
    1014
  • Tag / Fusion Protein
    • HA-NLS (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIIS1011 was a gift from Feng Zhang (Addgene plasmid # 238212 ; http://n2t.net/addgene:238212 ; RRID:Addgene_238212)
  • For your References section:

    TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses. Faure G, Saito M, Wilkinson ME, Quinones-Olvera N, Xu P, Flam-Shepherd D, Kim S, Reddy N, Zhu S, Evgeniou L, Koonin EV, Macrae RK, Zhang F. Science. 2025 Feb 27:eadv9789. doi: 10.1126/science.adv9789. 10.1126/science.adv9789 PubMed 40014690