pBR25 CFAP36 [L438E, K442E, A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)
(Plasmid
#238229)
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PurposeCRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E, A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBR25
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Vector typeCRISPR ; Donor
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCFAP36 [L438E, K442E, A493E, K495A]
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SpeciesC. reinhardtii
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GenBank IDCre09.g395250_4532
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Tag
/ Fusion Protein
- -3C-mStayGold-2Strep (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBR25 CFAP36 [L438E, K442E, A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii) was a gift from Alan Brown (Addgene plasmid # 238229 ; http://n2t.net/addgene:238229 ; RRID:Addgene_238229) -
For your References section:
A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia. Lange SM, Bennett JA, Eisert RJ, Brown A. Cell. 2025 Nov 13;188(23):6492-6508.e18. doi: 10.1016/j.cell.2025.07.043. Epub 2025 Aug 20. 10.1016/j.cell.2025.07.043 PubMed 40840444