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pBR25 IFT70 [A331R, A335R] crSpecR (C. reinhardtii)
(Plasmid #238230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238230 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBR25
  • Vector type
    CRISPR ; Donor
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IFT70 [A331R, A335R]
  • Species
    C. reinhardtii
  • GenBank ID
    Cre07.g342200_4532
  • Tag / Fusion Protein
    • None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBR25 IFT70 [A331R, A335R] crSpecR (C. reinhardtii) was a gift from Alan Brown (Addgene plasmid # 238230 ; http://n2t.net/addgene:238230 ; RRID:Addgene_238230)

  • For your References section:

    A conserved mechanism for the retrieval of polyubiquitinated proteins from cilia. Lange SM, Bennett JA, Eisert RJ, Brown A. Cell. 2025 Nov 13;188(23):6492-6508.e18. doi: 10.1016/j.cell.2025.07.043. Epub 2025 Aug 20. 10.1016/j.cell.2025.07.043 PubMed 40840444
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