Skip to main content
Addgene

pMH-SpAsp1
(Plasmid #238315)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238315 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pMH
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    asp1
  • Alt name
    PPIP5K
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    2763
  • Mutation
    SpAsp1
  • Entrez Gene
    asp1
  • Tag / Fusion Protein
    • His7-Tag, Strep-tag II, SUMO protein (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-SpAsp1 was a gift from Michael Hothorn (Addgene plasmid # 238315 ; http://n2t.net/addgene:238315 ; RRID:Addgene_238315)

  • For your References section:

    A small signaling domain controls PPIP5K phosphatase activity in phosphate homeostasis. Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, Vadas O, Panse VG, Fiedler D, Hothorn M. Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0. 10.1038/s41467-025-56937-0 PubMed 39966396
Related items: