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Addgene

pBB3-ScVip1-536-1107-K817S-D819A-S821A
(Plasmid #238326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBB3
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VIP1
  • Alt name
    PPIP5K
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1716
  • Mutation
    ScVip1-536-1107-K817S-D819A-S821A
  • Entrez Gene
    VIP1 (a.k.a. YLR410W)
  • Tag / Fusion Protein
    • His10-Tag, Twin-Strep-tag, TEV (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBB3-ScVip1-536-1107-K817S-D819A-S821A was a gift from Michael Hothorn (Addgene plasmid # 238326 ; http://n2t.net/addgene:238326 ; RRID:Addgene_238326)

  • For your References section:

    A small signaling domain controls PPIP5K phosphatase activity in phosphate homeostasis. Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, Vadas O, Panse VG, Fiedler D, Hothorn M. Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0. 10.1038/s41467-025-56937-0 PubMed 39966396
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