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Addgene

Ec FkP-dnaN(3E's)-pRSFDuet
(Plasmid #238344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E)
  • Species
    E. coli
  • Mutation
    R96E; R103E; R105E
  • Entrez Gene
    dnaN (a.k.a. b3701, ECK3693)
  • Tag / Fusion Protein
    • 3X FLAG+PKA kinase motif+PreScission Protease cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ec FkP-dnaN(3E's)-pRSFDuet was a gift from Michael O'Donnell (Addgene plasmid # 238344 ; http://n2t.net/addgene:238344 ; RRID:Addgene_238344)
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