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Amber-free pNL4-3 FLuc Env-RT-
(Plasmid #238415)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238415 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pNL4-3.Luc.R-E-
  • Total vector size (bp) 16395
  • Modifications to backbone
    There are two mutation sites in the Env gene: M147TAA and M149TAA.
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly luciferase
  • Species
    Synthetic
  • Insert Size (bp)
    1677
  • Mutation
    Alternation of amber stop codons "TAG" in the Luciferase gene (5014-5016) and the NES gene (6834-6836) to "TAA".
  • Entrez Gene
    LOC116160065 (a.k.a. PPYR_00001, LUC1, PpyLuc1, luc, luciferase)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Amber-free pNL4-3 FLuc Env-RT- was a gift from Maolin Lu (Addgene plasmid # 238415 ; http://n2t.net/addgene:238415 ; RRID:Addgene_238415)

  • For your References section:

    Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion. Xu W, Gonepudi NK, Lu M. STAR Protoc. 2025 Jul 26;6(3):103995. doi: 10.1016/j.xpro.2025.103995. 10.1016/j.xpro.2025.103995 PubMed 40716060
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