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Addgene

FLAG-GRP78-DeltaER
(Plasmid #238416)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9599
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    78-kDa glucose-regulated protein
  • Alt name
    GRP78
  • Alt name
    BiP
  • Alt name
    HSPA5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Mutation
    The first 18 amino acids which is the ER signal peptide is deleted.
  • GenBank ID
    NM_005347.5
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-GRP78-DeltaER was a gift from Amy Shiu Lee (Addgene plasmid # 238416 ; http://n2t.net/addgene:238416 ; RRID:Addgene_238416)

  • For your References section:

    Requirements for nuclear GRP78 transcriptional regulatory activities and interaction with nuclear GRP94. Liu Z, Ha DP, Lin LL, Qi L, Lee AS. J Biol Chem. 2025 Feb 28;301(4):108369. doi: 10.1016/j.jbc.2025.108369. 10.1016/j.jbc.2025.108369 PubMed 40024475
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