FLAG-GRP78-DeltaER
(Plasmid
#238416)
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PurposeExpresses GRP78 lacking the ER signal peptide in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238416 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecustom
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 9599
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name78-kDa glucose-regulated protein
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Alt nameGRP78
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Alt nameBiP
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Alt nameHSPA5
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SpeciesH. sapiens (human)
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Insert Size (bp)4200
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MutationThe first 18 amino acids which is the ER signal peptide is deleted.
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GenBank IDNM_005347.5
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Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-GRP78-DeltaER was a gift from Amy Shiu Lee (Addgene plasmid # 238416 ; http://n2t.net/addgene:238416 ; RRID:Addgene_238416) -
For your References section:
Requirements for nuclear GRP78 transcriptional regulatory activities and interaction with nuclear GRP94. Liu Z, Ha DP, Lin LL, Qi L, Lee AS. J Biol Chem. 2025 Feb 28;301(4):108369. doi: 10.1016/j.jbc.2025.108369. 10.1016/j.jbc.2025.108369 PubMed 40024475