CD44v-HA (pcDNA3)
(Plasmid
#238417)
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PurposeExpresses CD44, a single-pass transmembrane protein containing variable exons 3 to 10.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238417 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 7500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD44 containing exons 3 to 10
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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Entrez GeneCD44 (a.k.a. CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CD44v-HA (pcDNA3) was a gift from Amy Shiu Lee (Addgene plasmid # 238417 ; http://n2t.net/addgene:238417 ; RRID:Addgene_238417) -
For your References section:
GRP78 regulates CD44v membrane homeostasis and cell spreading in tamoxifen-resistant breast cancer. Tseng CC, Stanciauskas R, Zhang P, Woo D, Wu K, Kelly K, Gill PS, Yu M, Pinaud F, Lee AS. Life Sci Alliance. 2019 Aug 15;2(4):e201900377. doi: 10.26508/lsa.201900377. Print 2019 Aug. 10.26508/lsa.201900377 PubMed 31416894