FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1)
(Plasmid
#238429)
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PurposeExpresses GRP78 Full Length without UTRs fused to the FLAG Tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5500
- Total vector size (bp) 9599
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name78-kDa glucose-regulated protein
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Alt nameGRP78
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Alt nameBiP
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Alt nameHSPA5
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SpeciesH. sapiens (human)
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Insert Size (bp)4200
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MutationThe UTRs of the gene have been deleted.
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GenBank IDNM_005347.5
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Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-GRP78-No UTRs Wild-Type (pcDNA3.1) was a gift from Amy Shiu Lee (Addgene plasmid # 238429 ; http://n2t.net/addgene:238429 ; RRID:Addgene_238429) -
For your References section:
The COOH-Terminal Proline-Rich Region of GRP78 Is a Key Regulator of Its Cell Surface Expression and Viability of Tamoxifen-Resistant Breast Cancer Cells. Tseng CC, Zhang P, Lee AS. Neoplasia. 2019 Aug;21(8):837-848. doi: 10.1016/j.neo.2019.05.008. Epub 2019 Jul 12. 10.1016/j.neo.2019.05.008 PubMed 31306849