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Addgene

pcDNA3-P-SSP-AAA Mutant
(Plasmid #238432)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238432 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 5700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    amino acid 631-650 of AAA Mutant GRP78 preceded by a secretory and sorting peptide of α-melanocyte-stimulating hormone (SSP)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    100
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-P-SSP-AAA Mutant was a gift from Amy Shiu Lee (Addgene plasmid # 238432 ; http://n2t.net/addgene:238432 ; RRID:Addgene_238432)

  • For your References section:

    The COOH-Terminal Proline-Rich Region of GRP78 Is a Key Regulator of Its Cell Surface Expression and Viability of Tamoxifen-Resistant Breast Cancer Cells. Tseng CC, Zhang P, Lee AS. Neoplasia. 2019 Aug;21(8):837-848. doi: 10.1016/j.neo.2019.05.008. Epub 2019 Jul 12. 10.1016/j.neo.2019.05.008 PubMed 31306849
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