POC1553
(Plasmid
#238470)
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Purpose(Empty Backbone) pMOBC360_VL: Vector Left (VL) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMXBC
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Backbone manufacturerSelf-made
- Backbone size (bp) 7998
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gene Synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The DH10B strain should be used for expression of this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
POC1553 was a gift from Christopher A. O'Callaghan (Addgene plasmid # 238470 ; http://n2t.net/addgene:238470 ; RRID:Addgene_238470) -
For your References section:
UniClo: scarless hierarchical DNA assembly without sequence constraint. Flores-Fernandez CN, Lin D, Robins K, O'Callaghan CA. Nucleic Acids Res. 2025 Jun 20;53(12):gkaf548. doi: 10.1093/nar/gkaf548. 10.1093/nar/gkaf548 PubMed 40548934
Map uploaded by the depositor.
