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pLP-PH16_RS09790-pcepA-himar-chlorR
(Plasmid #238494)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238494 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom (R6K origin)
  • Vector type
    Bacterial Expression
  • Promoter BenM/PH16_RS09790

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    Himar transposase expression is under the regulation of the heat-inactivated CI857 repressor. Grow at 37˚C to express himar transposase in recipient strain.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLP-PH16_RS09790-pcepA-himar-chlorR was a gift from Farren Isaacs (Addgene plasmid # 238494 ; http://n2t.net/addgene:238494 ; RRID:Addgene_238494)

  • For your References section:

    Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems in Cupriavidus necator H16. Nakamura AK, Fulk EM, Johnson CW, Isaacs FJ. ACS Synth Biol. 2025 Mar 21;14(3):943-953. doi: 10.1021/acssynbio.4c00869. Epub 2025 Mar 6. 10.1021/acssynbio.4c00869 PubMed 40048245
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