PDZK1P1_pGCS1
(Plasmid
#238501)
-
PurposeIVT mRNA of human gene
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 238501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGCS1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePDZK1P1
-
SpeciesH. sapiens (human)
-
Entrez GenePDZK1P1 (a.k.a. PDZK1P2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made bySynthesized original insert through Twist Biosciences using transcript Iso-Seq data from ENCODE (GenBank ENCFF158KCA; ENCFF939EUU)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.26.615256 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PDZK1P1_pGCS1 was a gift from Megan Dennis (Addgene plasmid # 238501 ; http://n2t.net/addgene:238501 ; RRID:Addgene_238501) -
For your References section:
Gene expansions contributing to human brain evolution. Soto DC, Uribe-Salazar JM, Kaya G, Valdarrago R, Sekar A, Haghani NK, Hino K, La GN, Mariano NAF, Ingamells C, Baraban AE, Turner TN, Green ED, Simo S, Quon G, Andres AM, Dennis MY. bioRxiv [Preprint]. 2024 Sep 26:2024.09.26.615256. doi: 10.1101/2024.09.26.615256. 10.1101/2024.09.26.615256 PubMed 39386494