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Addgene

pMH_AtCK2alpha1_76-409_K146A_K148/149A_HC
(Plasmid #238516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH (pET-24d based)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtCK2alpha1
  • Species
    A. thaliana (mustard weed)
  • Mutation
    K146A_K148/149A
  • Entrez Gene
    CKA1 (a.k.a. ATCKA1, ATPK12D, CASEIN KINASE ALPHA 1, CKA1, K8K14.10, K8K14_10)
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH_AtCK2alpha1_76-409_K146A_K148/149A_HC was a gift from Michael Hothorn (Addgene plasmid # 238516 ; http://n2t.net/addgene:238516 ; RRID:Addgene_238516)
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