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pMH_MBP_AtCK2beta1_95-287
(Plasmid #238519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMH (pET-24d based)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtCK2beta1
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    CKB1 (a.k.a. casein kinase II beta chain 1, CASEIN KINASE II BETA SUBUNIT CKB1, CKB1, K14A3.3, K14A3_3)
  • Tag / Fusion Protein
    • 2x Strep, 6x His, MBP, N-term, TEV cleavable (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH_MBP_AtCK2beta1_95-287 was a gift from Michael Hothorn (Addgene plasmid # 238519 ; http://n2t.net/addgene:238519 ; RRID:Addgene_238519)
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