pLII-CRISPR for CKA4
(Plasmid
#238521)
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PurposePlant expression of Cas9 and gRNAs against A. thaliana CK2alpha4
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLII beta R 5-6
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtCK2alpha4
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SpeciesA. thaliana (mustard weed)
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Entrez GeneAT2G23070 (a.k.a. F21P24.13, F21P24_13)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLII-CRISPR for CKA4 was a gift from Michael Hothorn (Addgene plasmid # 238521 ; http://n2t.net/addgene:238521 ; RRID:Addgene_238521)