pLII-proUBI10_CKA3 KD K63M,N170D-FLAG_termNOS _OLE-RED
(Plasmid
#238523)
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PurposePlant expression of flag-tagged A. thaliana CK2alpha3 KD K63M,N170D; with OLE1-RED marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepLII beta R 5-6
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtCK2alpha3
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SpeciesA. thaliana (mustard weed)
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MutationK63M N170D
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Entrez GeneAT2G23080 (a.k.a. F21P24.14, F21P24_14)
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLII-proUBI10_CKA3 KD K63M,N170D-FLAG_termNOS _OLE-RED was a gift from Michael Hothorn (Addgene plasmid # 238523 ; http://n2t.net/addgene:238523 ; RRID:Addgene_238523)