pLIII CKBs CRISPR with guides at 1-2 and 2-3
(Plasmid
#238525)
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PurposePlant expression of Cas9 and gRNAs against A. thaliana CK2alpha3
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238525 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLIII
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtCK2alpha3
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SpeciesA. thaliana (mustard weed)
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Entrez GeneAT2G23080 (a.k.a. F21P24.14, F21P24_14)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIII CKBs CRISPR with guides at 1-2 and 2-3 was a gift from Michael Hothorn (Addgene plasmid # 238525 ; http://n2t.net/addgene:238525 ; RRID:Addgene_238525)