GE-0010-gRNA1-MpCKA
(Plasmid
#238526)
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PurposePlant expression of Cas9 and gRNA against M. polymorpha CK2 alpha
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMpGWB103
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Backbone manufacturerTakayuki Kohchi (Addgene plasmid # 68628)
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMpCKA
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Alt nameMp8g05810
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gRNA/shRNA sequencettaacgtgcagcggccca
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SpeciesM. polymorpha
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GE-0010-gRNA1-MpCKA was a gift from Michael Hothorn (Addgene plasmid # 238526 ; http://n2t.net/addgene:238526 ; RRID:Addgene_238526)