pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05
(Plasmid
#238556)
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PurposeA LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Intermediate (I) density. The GCaMP ORF can be swapped for your gene of interest.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238556 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHD
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Vector typeInsect Expression ; SPARC Plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP8m
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Alt name6xHis-GCaMP8m
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SpeciesSynthetic
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Insert Size (bp)1269
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Tag
/ Fusion Protein
- DsRed-Express (C terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byZhang & Looger (GCaMP8m), Isaacman-Beck & Clandinin (SPARC machinery)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05 was a gift from Tom Clandinin (Addgene plasmid # 238556 ; http://n2t.net/addgene:238556 ; RRID:Addgene_238556) -
For your References section:
Infrequent strong connections constrain connectomic predictions of neuronal function. TA Currier, TR Clandinin. Cell, CELL-D-24-04567, CELL13979