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NanoBRET BiBRET Vector, HaloTag-KRAS(G12D)_BRAF-NanoLuc
(Plasmid #238563)

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Item Catalog # Description Quantity Price (USD)
Plasmid 238563 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *

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Backbone

  • Vector backbone
    pFC223C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector and pFN222K HaloTag(R) CMV-Blast BiBRET Vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    KRAS
  • Species
    H. sapiens (human)
  • Mutation
    G12D
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter CMV
  • Tag / Fusion Protein
    • HaloTag (R) (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Other

Gene/Insert 2

  • Gene/Insert name
    BRAF
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
  • Tag / Fusion Protein
    • NanoLuc (R) (C terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NanoBRET BiBRET Vector, HaloTag-KRAS(G12D)_BRAF-NanoLuc was a gift from Promega Corporation (Addgene plasmid # 238563)
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