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Addgene

NanoBRET Assay, TEAD2 / YAP Bidirectional Vector
(Plasmid #238669)

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Item Catalog # Description Quantity Price (USD)
Plasmid 238669 Standard format: Plasmid sent in bacteria as agar stab 1 $89 *

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Backbone

  • Vector backbone
    pFC221K HaloTag(R) CMV-Blast BiBRET Vector and pFN224C NanoLuc(R) CMV-Neo BiBRET-Balanced Vector
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TEAD2
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    TEAD2 (a.k.a. ETF, TEAD-2, TEF-4, TEF4)
  • Promoter CMV
  • Tag / Fusion Protein
    • NanoLuc (R) (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Other

Gene/Insert 2

  • Gene/Insert name
    YAP
  • Species
    H. sapiens (human)
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Tag / Fusion Protein
    • HaloTag (R) (C terminal on backbone)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NanoBRET Assay, TEAD2 / YAP Bidirectional Vector was a gift from Promega Corporation (Addgene plasmid # 238669)
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