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Addgene

twinStrep_HA_aMFx1 (4a)
(Plasmid #238905)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238905 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    High Copy Chloramphenicol
  • Backbone manufacturer
    TWIST bioscience
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    twinStrep_HA_aMFx1
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are two twinStreps in this plasmid, and the depositor confirms that this does not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    twinStrep_HA_aMFx1 (4a) was a gift from Joseph Brock (Addgene plasmid # 238905 ; http://n2t.net/addgene:238905 ; RRID:Addgene_238905)

  • For your References section:

    High-throughput optimisation of protein secretion in yeast via an engineered biosensor. Cleaver A, Luo R, Smith OB, Murphy L, Schwessinger B, Brock J. Trends Biotechnol. 2025 Apr;43(4):838-867. doi: 10.1016/j.tibtech.2024.11.010. Epub 2024 Dec 13. 10.1016/j.tibtech.2024.11.010 PubMed 39674781
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