pAAV_CaMKII-RFP-p2A-DAAO-NES
(Plasmid
#238918)
-
PurposeMammalian expression of DAAO with a nuclear export signal and RFP as a reporter in excitatory neurons
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 238918 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepAAV-mDlx-GFP-Fishell-1
-
Backbone manufacturerAddgene (https://www.addgene.org/83900/)
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRFP-p2A-DAAO-NES
-
SpeciesSynthetic
-
Insert Size (bp)1922
- Promoter CaMKIIalfa
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV_CaMKII-RFP-p2A-DAAO-NES was a gift from Vsevolod Belousov (Addgene plasmid # 238918 ; http://n2t.net/addgene:238918 ; RRID:Addgene_238918) -
For your References section:
Chemogenetic emulation of intraneuronal oxidative stress affects synaptic plasticity. Kalinichenko AL, Jappy D, Solius GM, Maltsev DI, Bogdanova YA, Mukhametshina LF, Sokolov RA, Moshchenko AA, Shaydurov VA, Rozov AV, Podgorny OV, Belousov VV. Redox Biol. 2023 Apr;60:102604. doi: 10.1016/j.redox.2023.102604. Epub 2023 Jan 10. 10.1016/j.redox.2023.102604 PubMed 36640726