pAAV_CaMKII-TRPV1-flag
(Plasmid
#238924)
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PurposeExpression of the human TRPV1 channel with a Flag-tag in excitatory neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV-HyPer-DAAO-NES
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Backbone manufacturerThomas Michel (Addgene #119164)
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaMKII-TRPV1-flag
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SpeciesSynthetic
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Insert Size (bp)2590
- Promoter CaMKIIalfa
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Tag
/ Fusion Protein
- flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_CaMKII-TRPV1-flag was a gift from Vsevolod Belousov (Addgene plasmid # 238924 ; http://n2t.net/addgene:238924 ; RRID:Addgene_238924) -
For your References section:
Human TRPV1 is an efficient thermogenetic actuator for chronic neuromodulation. Maltsev DI, Solotenkov MA, Mukhametshina LF, Sokolov RA, Solius GM, Jappy D, Tsopina AS, Fedotov IV, Lanin AA, Fedotov AB, Krut' VG, Ermakova YG, Moshchenko AA, Rozov A, Zheltikov AM, Podgorny OV, Belousov VV. Cell Mol Life Sci. 2024 Oct 25;81(1):437. doi: 10.1007/s00018-024-05475-x. 10.1007/s00018-024-05475-x PubMed 39448456