pC1-HyPerFLEX-ERlum
(Plasmid
#238941)
-
PurposeMammalian expression of HyPerFLEX targeted to the luminal side of the endoplasmic reticulum membranes
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 238941 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepC1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameHyPerFLEX-ERlum (Calreticulin signal sequence-HyPerFLEX-KDEL)
-
SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pC1-HyPerFLEX-ERlum was a gift from Vsevolod Belousov (Addgene plasmid # 238941 ; http://n2t.net/addgene:238941 ; RRID:Addgene_238941) -
For your References section:
A color-tailored fluorogenic sensor for hydrogen peroxide. Potekhina ES, Bass DI, Ezerina D, Fleckenstein DD, Chebotarev AS, Sysoeva VA, Maltsev DI, Pak VV, Moshchenko AA, Sokolov AI, Myasnyanko IN, Baranov MS, Fedotov AB, Zheltikov AM, Lanin AA, Messens J, Belousov VV. Nat Chem Biol. 2025 Oct 16. doi: 10.1038/s41589-025-02036-6. 10.1038/s41589-025-02036-6 PubMed 41102408