pAAV_GFAP_HyPer7_mito
(Plasmid
#238966)
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PurposeMammalian astrocytes HyPer7 expression targeted to the mitochondrial matrix
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepAAV9
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHyPer7
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SpeciesSynthetic
- Promoter GFAP
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Tag
/ Fusion Protein
- 3x Cytochrome C Oxidase Subunit VIII presequense (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also named pAAV9_GFAP_HyPer7_mito in Kotova et al. 2023.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_GFAP_HyPer7_mito was a gift from Dmitry Bilan (Addgene plasmid # 238966 ; http://n2t.net/addgene:238966 ; RRID:Addgene_238966) -
For your References section:
Hyperglycemia exacerbates ischemic stroke not through increased generation of hydrogen peroxide. Kotova DA, Ivanova AD, Pochechuev MS, Kelmanson IV, Khramova YV, Tiaglik A, Sudoplatov MA, Trifonova AP, Fedotova A, Morozova K, Katrukha VA, Sergeeva AD, Raevskii RI, Pestriakova MP, Solotenkov MA, Stepanov EA, Tsopina AS, Moshchenko AA, Shestopalova M, Zalygin A, Fedotov IV, Fedotov AB, Oleinikov V, Belousov VV, Semyanov A, Brazhe N, Zheltikov AM, Bilan DS. Free Radic Biol Med. 2023 Nov 1;208:153-164. doi: 10.1016/j.freeradbiomed.2023.08.004. Epub 2023 Aug 4. 10.1016/j.freeradbiomed.2023.08.004 PubMed 37543166