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Addgene

BtA2
(Plasmid #238982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2800
  • Vector type
    In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-2 long
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1400
  • Mutation
    none
  • Entrez Gene
    ARRB2
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BtA2 was a gift from Jeffrey Benovic & Vsevolod Gurevich (Addgene plasmid # 238982 ; http://n2t.net/addgene:238982 ; RRID:Addgene_238982)

  • For your References section:

    Polypeptide variants of beta-arrestin and arrestin3. Sterne-Marr R, Gurevich VV, Goldsmith P, Bodine RC, Sanders C, Donoso LA, Benovic JL. J Biol Chem. 1993 Jul 25;268(21):15640-8. PubMed 8340388
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