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Addgene

pVeBtA2d7
(Plasmid #238989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-2 long
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2000
  • Mutation
    deleted residues 174, 176, 177, 181-184
  • Entrez Gene
    ARRB2
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVeBtA2d7 was a gift from Vsevolod Gurevich (Addgene plasmid # 238989 ; http://n2t.net/addgene:238989 ; RRID:Addgene_238989)

  • For your References section:

    Arrestin mobilizes signaling proteins to the cytoskeleton and redirects their activity. Hanson SM, Cleghorn WM, Francis DJ, Vishnivetskiy SA, Raman D, Song X, Nair KS, Slepak VZ, Klug CS, Gurevich VV. J Mol Biol. 2007 Apr 27;368(2):375-87. doi: 10.1016/j.jmb.2007.02.053. Epub 2007 Feb 22. 10.1016/j.jmb.2007.02.053 PubMed 17359998
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