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Addgene

BtA3CL
(Plasmid #238992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2800
  • Vector type
    In vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-3 short
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1400
  • Mutation
    C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C270S, and C409S
  • Entrez Gene
    ARR3
  • Promoter SP6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BtA3CL was a gift from Vsevolod Gurevich (Addgene plasmid # 238992 ; http://n2t.net/addgene:238992 ; RRID:Addgene_238992)

  • For your References section:

    Ubiquitin ligase parkin promotes Mdm2-arrestin interaction but inhibits arrestin ubiquitination. Ahmed MR, Zhan X, Song X, Kook S, Gurevich VV, Gurevich EV. Biochemistry. 2011 May 10;50(18):3749-63. doi: 10.1021/bi200175q. Epub 2011 Apr 12. 10.1021/bi200175q PubMed 21466165
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