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pEND-Y11
(Plasmid #239000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239000 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCOLA
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Circuit with sfGFP expression only in the absence of Ara (-Ara: ON, +Ara: OFF)
  • Species
    Escherichia coli
  • Mutation
    WT

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank file with original annotations can be downloaded from the "Resource Information" section. Please note that, in addition, plasmid pJ1996_v2 is needed for the circuit encoded on this plasmid to operate as designed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEND-Y11 was a gift from Javier Santos-Moreno (Addgene plasmid # 239000 ; http://n2t.net/addgene:239000 ; RRID:Addgene_239000)

  • For your References section:

    Critical Analysis of Preprints and Inquiry-Based Lessons Improve the Synthetic Biology Learning Experience. Nevot G, Guell M, Santos-Moreno J. ACS Synth Biol. 2025 Aug 15;14(8):2878-2884. doi: 10.1021/acssynbio.5c00014. 10.1021/acssynbio.5c00014 PubMed 40814254
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