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Addgene

pET29b-Rhobin2
(Plasmid #239018)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239018 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RhoBin2
  • Species
    Synthetic
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encoding de novo Rhodamine binder protein Rhobin2 with N-terminal 6xHisTag-TEV protease cleavage sequence . Please visit https://doi.org/10.1101/2025.06.24.661379 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-Rhobin2 was a gift from William DeGrado & Bo Huang (Addgene plasmid # 239018 ; http://n2t.net/addgene:239018 ; RRID:Addgene_239018)

  • For your References section:

    De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy. Chen Y, Yserentant K, Hong K, Kuang Y, Bhowmick A, Charles-Orszag A, Lord SJ, Lu L, Hou K, Mann SI, Grimm JB, Lavis LD, Mullins RD, DeGrado WF, Huang B. bioRxiv 2025.06.24.661379 10.1101/2025.06.24.661379
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