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pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS
(Plasmid #239020)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIST CMV OriP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rhobin2-H2B
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    H2BC21 (a.k.a. GL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE)
  • Promoter CMV promoter
  • Tags / Fusion Proteins
    • mNeonGreen3K (C terminal on insert)
    • TagBFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Encoding Rhobin2-H2B-mNeonGreen3K fusion protein for Rhobin-dye labeling validation with mNeonGreen3K signal, and also encoding NLS-mTagBFP-2xNLS as a nuclear labeling marker . Please visit https://doi.org/10.1101/2025.06.24.661379 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS was a gift from William DeGrado & Bo Huang (Addgene plasmid # 239020 ; http://n2t.net/addgene:239020 ; RRID:Addgene_239020)

  • For your References section:

    De novo designed bright, hyperstable rhodamine binders for fluorescence microscopy. Chen Y, Yserentant K, Hong K, Kuang Y, Bhowmick A, Charles-Orszag A, Lord SJ, Lu L, Hou K, Mann SI, Grimm JB, Lavis LD, Mullins RD, DeGrado WF, Huang B. bioRxiv 2025.06.24.661379 10.1101/2025.06.24.661379
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