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p15GFPkv
(Plasmid #239039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239039 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pmaxCloning
  • Backbone manufacturer
    Lonza
  • Total vector size (bp) 3599
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p15GFPKv-NES
  • Alt name
    +15GFPKv
  • Alt name
    +15GFP-Kv
  • Species
    Synthetic
  • Insert Size (bp)
    802
  • Mutation
    All surface-exposed Arg residues changed to Lysine
  • Promoter CMV

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer aggaggccaatagaaactggg
  • 3′ sequencing primer gaatgcaattgttgttgttaacttgt
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p15GFPkv was a gift from Felipe Quiroz (Addgene plasmid # 239039 ; http://n2t.net/addgene:239039 ; RRID:Addgene_239039)

  • For your References section:

    Genetically-Encoded Phase Separation Sensors Enable High-Fidelity Live-Cell Probing of Biomolecular Condensates. Avecilla ARC, Thomas J, Quiroz FG. ACS Sens. 2025 Mar 28;10(3):1857-1869. doi: 10.1021/acssensors.4c02851. Epub 2025 Feb 23. 10.1021/acssensors.4c02851 PubMed 39987501
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