AAV-DIO-beta-Endorphin
(Plasmid
#239043)
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PurposeOverexpression of beta endorphin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV[FLEXon]
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Backbone manufacturerVector Builder
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebeta-Endorphin
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SpeciesM. musculus (mouse)
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Insert Size (bp)336
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Entrez GenePomc (a.k.a. ACTH, BE, Beta-LPH, Clip, Gamma-LPH, Npp, Pomc-1, Pomc1, alpha-MSH, alphaMSH, beta-MSH, gamma-MSH)
- Promoter CAG
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer CAG-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-DIO-beta-Endorphin was a gift from Qingchun Tong (Addgene plasmid # 239043 ; http://n2t.net/addgene:239043 ; RRID:Addgene_239043) -
For your References section:
Lateral septum as a melanocortin downstream site in obesity development. Xu Y, Jiang Z, Li H, Cai J, Jiang Y, Otiz-Guzman J, Xu Y, Arenkiel BR, Tong Q. Cell Rep. 2023 May 30;42(5):112502. doi: 10.1016/j.celrep.2023.112502. Epub 2023 May 11. 10.1016/j.celrep.2023.112502 PubMed 37171957