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pBL071
(Plasmid #239089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239089 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    COLE1
  • Backbone size w/o insert (bp) 2590
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The S2060 strain should be used for expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cCadC-AB42
  • Species
    Synthetic
  • Insert Size (bp)
    609
  • Promoter PTet
  • Tag / Fusion Protein
    • cCadC fused to AB42

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer CGCTTACAGACAAGCTGTGACCGTC
  • 3′ sequencing primer AGCCACGATCGACATTGATCTGGCTATCTTGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.11.642651 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBL071 was a gift from Tina Wang (Addgene plasmid # 239089 ; http://n2t.net/addgene:239089 ; RRID:Addgene_239089)

  • For your References section:

    A genetically encoded selection for amyloid-beta oligomer binders. Lee B, Mannone JA, Wang T. Nat Chem Biol. 2025 Jul 15. doi: 10.1038/s41589-025-01975-4. 10.1038/s41589-025-01975-4 PubMed 40664790
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