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pCAD123_hIrg1_N152K,M154I_pvp008
(Plasmid #239118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239118 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCOLADuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3519
  • Total vector size (bp) 4893
  • Modifications to backbone
    N-terminal StrepTag and TEV site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    cis-aconitate decarboxylase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1374
  • Mutation
    Deleted amino acids 1-3 and 462-end. Mutated N152K and M154I
  • GenBank ID
    NM_001258406
  • Entrez Gene
    ACOD1 (a.k.a. CAD, IRG1)
  • Tag / Fusion Protein
    • StrepTag and TEV site (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Method for purification of the human enzyme cis-aconitate decarboxylase (ACOD1) from this plasmid in recombinant E. coli cells: http://dx.doi.org/10.13140/RG.2.2.12579.58405

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAD123_hIrg1_N152K,M154I_pvp008 was a gift from Konrad Buessow (Addgene plasmid # 239118 ; http://n2t.net/addgene:239118 ; RRID:Addgene_239118)

  • For your References section:

    Amino acid positions near the active site determine the reduced activity of human ACOD1 compared to murine ACOD1. Chen F, Yalcin I, Zhao M, Chen C, Blankenfeldt W, Pessler F, Bussow K. Sci Rep. 2023 Jun 26;13(1):10360. doi: 10.1038/s41598-023-37373-w. 10.1038/s41598-023-37373-w PubMed 37365251
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