pLS414
(Plasmid
#239125)
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Purpose(Empty Backbone) Minimal centromeric shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLS414
- Backbone size (bp) 3183
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Vector typeBacterial Expression, Yeast Expression ; Centromeric
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Cloning Information
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLS414 was a gift from Sahand Jamal Rahi (Addgene plasmid # 239125 ; http://n2t.net/addgene:239125 ; RRID:Addgene_239125)
Map uploaded by the depositor.
