pLS400_YCR051W
(Plasmid
#239129)
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Purpose(Empty Backbone) Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMX
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLS400
- Backbone size (bp) 3804
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Modifications to backboneSequences from the non-essential gene YCR051W have been introduced into the MCS of pLS400 for single-copy genomic integration.
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Vector typeBacterial Expression, Yeast Expression ; Integrating
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.05.622133 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLS400_YCR051W was a gift from Sahand Jamal Rahi (Addgene plasmid # 239129 ; http://n2t.net/addgene:239129 ; RRID:Addgene_239129)
Map uploaded by the depositor.
